competitive elisa meaning in Chinese
竞争性
竞争elisa
Examples
- Polyclonal antibody to butachlor was prepared from rabbits immunized with butachlor - bsa conjugate , and a competitive elisa was developed for the detection of the butachlor a major component in a herbicide within the range of 110
Mg ml之间,且和化学结构相似的甲草胺和乙草胺无交叉反应。该方法也可用于检测稻田水样中丁草胺的含量。 - Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen
El旧a拐成惴现2个克隆的可容牲mg7seem明显的抗原结合舌队其a分别为0 832和0 912 ,均高出附性对照( 0 - Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides , we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library , which may be another form of antagonist for tnfa , and the mimotopes were identified by sandwich elisa . after 3 rounds of screening , we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa , and the results showed strongly binding . the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c
在对噬菌体环七肽库进行三轮亲和性筛选后,随机挑选20个噬菌体克隆, elisa鉴定出9个阳性克隆,经dna测序推出三种氨基酸序列: c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ,其中优势克隆序列为c - rrpaqsg - c ;鉴定结果显示阳性克隆能够与tnf受体结合,并且能够阻断tnf与受体的结合,提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性,为tnfa表位模拟肽。 - Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen , the positive dones possessing apparent inhibitory effect were singled out for later use
X阳性克到驶知烘扳原kbbjg )浊对阳性克隆进行限制陇卧赐析( uwi和hedlll )鉴定三用竞争elisatoljmg7重组噬菌体抗体性克隆对mg7单扶与其相应抗原结合忙的喇率,从中j ) ed出对mg7单抗与期眩抗原结合有抑余j效应的克隆用于进一涉研究。